quantile-quantile pplot plot (qq plot) Search Results


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PD-L1 served as a novel target in HJB-suppressed lung cancer. (A) STAT3 expression in HCC827 cells detected by <t>qPCR</t> after HJB (0, 50, 100 µM) treatment; (B) PD-L1 expression in HCC827 cells detected by qPCR after using small interfering RNA targeted STAT3; (C) PD-L1 expression in LUAD cells and normal lung fibroblast cells detected by qPCR; (D) IHC analysis of PD-L1 expression downloaded from the HPA ( www.proteinatlas.org ), scale bar, 200 µm; (E) Western blot analysis of PD-L1 expression after HJB (0, 50, 100 µM) treatment; (F) the efficiency of PD-L1 knockdown detected by qPCR; (G) cell growth of Lewis after PD-L1 knockdown and HJB (50 µM) treatment; (H) growth of HCC827 cells after PD-L1 knockdown and HJB (50 µM) treatment. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; n.s., not significant. HJB, 17-hydroxy-jolkinolide B; qPCR, quantitative polymerase chain reaction; NC, negative control; PD-L1, programmed death ligand 1; Sh-PD-L1, short hairpin RNA targeting PD-L1; SiPD-L1, small interfering RNA targeting PD-L1; Si-STAT3, small interfering RNA targeting PD-L1; GAPDH, glyceraldehyde phosphate dehydrogenase; IHC, immunohistochemistry; HPA, Human Protein Atlas; NC-HCC827, negative control of small interfering RNA used in HCC827 cells; Si-HCC827, small interfering RNA targeting PD-L1 used in HCC827 cells; NC-Lewis, negative control of short hairpin RNA targeting PD-L1 used in Lewis cells; shRNA-Lewis, short hairpin RNA targeting PD-L1 used in Lewis cells.
Mycawaytm Mycoplasma Real Time Qpcr Detection Kit, supplied by Yesen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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LabCorp rt-qpcr mycoplasma contamination detection service
PD-L1 served as a novel target in HJB-suppressed lung cancer. (A) STAT3 expression in HCC827 cells detected by <t>qPCR</t> after HJB (0, 50, 100 µM) treatment; (B) PD-L1 expression in HCC827 cells detected by qPCR after using small interfering RNA targeted STAT3; (C) PD-L1 expression in LUAD cells and normal lung fibroblast cells detected by qPCR; (D) IHC analysis of PD-L1 expression downloaded from the HPA ( www.proteinatlas.org ), scale bar, 200 µm; (E) Western blot analysis of PD-L1 expression after HJB (0, 50, 100 µM) treatment; (F) the efficiency of PD-L1 knockdown detected by qPCR; (G) cell growth of Lewis after PD-L1 knockdown and HJB (50 µM) treatment; (H) growth of HCC827 cells after PD-L1 knockdown and HJB (50 µM) treatment. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; n.s., not significant. HJB, 17-hydroxy-jolkinolide B; qPCR, quantitative polymerase chain reaction; NC, negative control; PD-L1, programmed death ligand 1; Sh-PD-L1, short hairpin RNA targeting PD-L1; SiPD-L1, small interfering RNA targeting PD-L1; Si-STAT3, small interfering RNA targeting PD-L1; GAPDH, glyceraldehyde phosphate dehydrogenase; IHC, immunohistochemistry; HPA, Human Protein Atlas; NC-HCC827, negative control of small interfering RNA used in HCC827 cells; Si-HCC827, small interfering RNA targeting PD-L1 used in HCC827 cells; NC-Lewis, negative control of short hairpin RNA targeting PD-L1 used in Lewis cells; shRNA-Lewis, short hairpin RNA targeting PD-L1 used in Lewis cells.
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Genechem mycoplasma qpcr detection kit
PD-L1 served as a novel target in HJB-suppressed lung cancer. (A) STAT3 expression in HCC827 cells detected by <t>qPCR</t> after HJB (0, 50, 100 µM) treatment; (B) PD-L1 expression in HCC827 cells detected by qPCR after using small interfering RNA targeted STAT3; (C) PD-L1 expression in LUAD cells and normal lung fibroblast cells detected by qPCR; (D) IHC analysis of PD-L1 expression downloaded from the HPA ( www.proteinatlas.org ), scale bar, 200 µm; (E) Western blot analysis of PD-L1 expression after HJB (0, 50, 100 µM) treatment; (F) the efficiency of PD-L1 knockdown detected by qPCR; (G) cell growth of Lewis after PD-L1 knockdown and HJB (50 µM) treatment; (H) growth of HCC827 cells after PD-L1 knockdown and HJB (50 µM) treatment. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; n.s., not significant. HJB, 17-hydroxy-jolkinolide B; qPCR, quantitative polymerase chain reaction; NC, negative control; PD-L1, programmed death ligand 1; Sh-PD-L1, short hairpin RNA targeting PD-L1; SiPD-L1, small interfering RNA targeting PD-L1; Si-STAT3, small interfering RNA targeting PD-L1; GAPDH, glyceraldehyde phosphate dehydrogenase; IHC, immunohistochemistry; HPA, Human Protein Atlas; NC-HCC827, negative control of small interfering RNA used in HCC827 cells; Si-HCC827, small interfering RNA targeting PD-L1 used in HCC827 cells; NC-Lewis, negative control of short hairpin RNA targeting PD-L1 used in Lewis cells; shRNA-Lewis, short hairpin RNA targeting PD-L1 used in Lewis cells.
Mycoplasma Qpcr Detection Kit, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ingenetix gmbh real-time pcr bactoreal kit mycoplasma hyorhinis
PD-L1 served as a novel target in HJB-suppressed lung cancer. (A) STAT3 expression in HCC827 cells detected by <t>qPCR</t> after HJB (0, 50, 100 µM) treatment; (B) PD-L1 expression in HCC827 cells detected by qPCR after using small interfering RNA targeted STAT3; (C) PD-L1 expression in LUAD cells and normal lung fibroblast cells detected by qPCR; (D) IHC analysis of PD-L1 expression downloaded from the HPA ( www.proteinatlas.org ), scale bar, 200 µm; (E) Western blot analysis of PD-L1 expression after HJB (0, 50, 100 µM) treatment; (F) the efficiency of PD-L1 knockdown detected by qPCR; (G) cell growth of Lewis after PD-L1 knockdown and HJB (50 µM) treatment; (H) growth of HCC827 cells after PD-L1 knockdown and HJB (50 µM) treatment. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; n.s., not significant. HJB, 17-hydroxy-jolkinolide B; qPCR, quantitative polymerase chain reaction; NC, negative control; PD-L1, programmed death ligand 1; Sh-PD-L1, short hairpin RNA targeting PD-L1; SiPD-L1, small interfering RNA targeting PD-L1; Si-STAT3, small interfering RNA targeting PD-L1; GAPDH, glyceraldehyde phosphate dehydrogenase; IHC, immunohistochemistry; HPA, Human Protein Atlas; NC-HCC827, negative control of small interfering RNA used in HCC827 cells; Si-HCC827, small interfering RNA targeting PD-L1 used in HCC827 cells; NC-Lewis, negative control of short hairpin RNA targeting PD-L1 used in Lewis cells; shRNA-Lewis, short hairpin RNA targeting PD-L1 used in Lewis cells.
Real Time Pcr Bactoreal Kit Mycoplasma Hyorhinis, supplied by Ingenetix gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher mycoseq mycoplasma real-time pcr
PD-L1 served as a novel target in HJB-suppressed lung cancer. (A) STAT3 expression in HCC827 cells detected by <t>qPCR</t> after HJB (0, 50, 100 µM) treatment; (B) PD-L1 expression in HCC827 cells detected by qPCR after using small interfering RNA targeted STAT3; (C) PD-L1 expression in LUAD cells and normal lung fibroblast cells detected by qPCR; (D) IHC analysis of PD-L1 expression downloaded from the HPA ( www.proteinatlas.org ), scale bar, 200 µm; (E) Western blot analysis of PD-L1 expression after HJB (0, 50, 100 µM) treatment; (F) the efficiency of PD-L1 knockdown detected by qPCR; (G) cell growth of Lewis after PD-L1 knockdown and HJB (50 µM) treatment; (H) growth of HCC827 cells after PD-L1 knockdown and HJB (50 µM) treatment. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; n.s., not significant. HJB, 17-hydroxy-jolkinolide B; qPCR, quantitative polymerase chain reaction; NC, negative control; PD-L1, programmed death ligand 1; Sh-PD-L1, short hairpin RNA targeting PD-L1; SiPD-L1, small interfering RNA targeting PD-L1; Si-STAT3, small interfering RNA targeting PD-L1; GAPDH, glyceraldehyde phosphate dehydrogenase; IHC, immunohistochemistry; HPA, Human Protein Atlas; NC-HCC827, negative control of small interfering RNA used in HCC827 cells; Si-HCC827, small interfering RNA targeting PD-L1 used in HCC827 cells; NC-Lewis, negative control of short hairpin RNA targeting PD-L1 used in Lewis cells; shRNA-Lewis, short hairpin RNA targeting PD-L1 used in Lewis cells.
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ATCC quantitative mycoplasma genomic dna
PD-L1 served as a novel target in HJB-suppressed lung cancer. (A) STAT3 expression in HCC827 cells detected by <t>qPCR</t> after HJB (0, 50, 100 µM) treatment; (B) PD-L1 expression in HCC827 cells detected by qPCR after using small interfering RNA targeted STAT3; (C) PD-L1 expression in LUAD cells and normal lung fibroblast cells detected by qPCR; (D) IHC analysis of PD-L1 expression downloaded from the HPA ( www.proteinatlas.org ), scale bar, 200 µm; (E) Western blot analysis of PD-L1 expression after HJB (0, 50, 100 µM) treatment; (F) the efficiency of PD-L1 knockdown detected by qPCR; (G) cell growth of Lewis after PD-L1 knockdown and HJB (50 µM) treatment; (H) growth of HCC827 cells after PD-L1 knockdown and HJB (50 µM) treatment. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; n.s., not significant. HJB, 17-hydroxy-jolkinolide B; qPCR, quantitative polymerase chain reaction; NC, negative control; PD-L1, programmed death ligand 1; Sh-PD-L1, short hairpin RNA targeting PD-L1; SiPD-L1, small interfering RNA targeting PD-L1; Si-STAT3, small interfering RNA targeting PD-L1; GAPDH, glyceraldehyde phosphate dehydrogenase; IHC, immunohistochemistry; HPA, Human Protein Atlas; NC-HCC827, negative control of small interfering RNA used in HCC827 cells; Si-HCC827, small interfering RNA targeting PD-L1 used in HCC827 cells; NC-Lewis, negative control of short hairpin RNA targeting PD-L1 used in Lewis cells; shRNA-Lewis, short hairpin RNA targeting PD-L1 used in Lewis cells.
Quantitative Mycoplasma Genomic Dna, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beyotime mycoplasma qpcr detection kit
PD-L1 served as a novel target in HJB-suppressed lung cancer. (A) STAT3 expression in HCC827 cells detected by <t>qPCR</t> after HJB (0, 50, 100 µM) treatment; (B) PD-L1 expression in HCC827 cells detected by qPCR after using small interfering RNA targeted STAT3; (C) PD-L1 expression in LUAD cells and normal lung fibroblast cells detected by qPCR; (D) IHC analysis of PD-L1 expression downloaded from the HPA ( www.proteinatlas.org ), scale bar, 200 µm; (E) Western blot analysis of PD-L1 expression after HJB (0, 50, 100 µM) treatment; (F) the efficiency of PD-L1 knockdown detected by qPCR; (G) cell growth of Lewis after PD-L1 knockdown and HJB (50 µM) treatment; (H) growth of HCC827 cells after PD-L1 knockdown and HJB (50 µM) treatment. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; n.s., not significant. HJB, 17-hydroxy-jolkinolide B; qPCR, quantitative polymerase chain reaction; NC, negative control; PD-L1, programmed death ligand 1; Sh-PD-L1, short hairpin RNA targeting PD-L1; SiPD-L1, small interfering RNA targeting PD-L1; Si-STAT3, small interfering RNA targeting PD-L1; GAPDH, glyceraldehyde phosphate dehydrogenase; IHC, immunohistochemistry; HPA, Human Protein Atlas; NC-HCC827, negative control of small interfering RNA used in HCC827 cells; Si-HCC827, small interfering RNA targeting PD-L1 used in HCC827 cells; NC-Lewis, negative control of short hairpin RNA targeting PD-L1 used in Lewis cells; shRNA-Lewis, short hairpin RNA targeting PD-L1 used in Lewis cells.
Mycoplasma Qpcr Detection Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


PD-L1 served as a novel target in HJB-suppressed lung cancer. (A) STAT3 expression in HCC827 cells detected by qPCR after HJB (0, 50, 100 µM) treatment; (B) PD-L1 expression in HCC827 cells detected by qPCR after using small interfering RNA targeted STAT3; (C) PD-L1 expression in LUAD cells and normal lung fibroblast cells detected by qPCR; (D) IHC analysis of PD-L1 expression downloaded from the HPA ( www.proteinatlas.org ), scale bar, 200 µm; (E) Western blot analysis of PD-L1 expression after HJB (0, 50, 100 µM) treatment; (F) the efficiency of PD-L1 knockdown detected by qPCR; (G) cell growth of Lewis after PD-L1 knockdown and HJB (50 µM) treatment; (H) growth of HCC827 cells after PD-L1 knockdown and HJB (50 µM) treatment. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; n.s., not significant. HJB, 17-hydroxy-jolkinolide B; qPCR, quantitative polymerase chain reaction; NC, negative control; PD-L1, programmed death ligand 1; Sh-PD-L1, short hairpin RNA targeting PD-L1; SiPD-L1, small interfering RNA targeting PD-L1; Si-STAT3, small interfering RNA targeting PD-L1; GAPDH, glyceraldehyde phosphate dehydrogenase; IHC, immunohistochemistry; HPA, Human Protein Atlas; NC-HCC827, negative control of small interfering RNA used in HCC827 cells; Si-HCC827, small interfering RNA targeting PD-L1 used in HCC827 cells; NC-Lewis, negative control of short hairpin RNA targeting PD-L1 used in Lewis cells; shRNA-Lewis, short hairpin RNA targeting PD-L1 used in Lewis cells.

Journal: Journal of Thoracic Disease

Article Title: 17-hydroxy-jolkinolide B potentiated CTLA4ab therapy through targeting tumor suppression and immune activation by downregulating PD-L1 expression in lung adenocarcinoma

doi: 10.21037/jtd-24-781

Figure Lengend Snippet: PD-L1 served as a novel target in HJB-suppressed lung cancer. (A) STAT3 expression in HCC827 cells detected by qPCR after HJB (0, 50, 100 µM) treatment; (B) PD-L1 expression in HCC827 cells detected by qPCR after using small interfering RNA targeted STAT3; (C) PD-L1 expression in LUAD cells and normal lung fibroblast cells detected by qPCR; (D) IHC analysis of PD-L1 expression downloaded from the HPA ( www.proteinatlas.org ), scale bar, 200 µm; (E) Western blot analysis of PD-L1 expression after HJB (0, 50, 100 µM) treatment; (F) the efficiency of PD-L1 knockdown detected by qPCR; (G) cell growth of Lewis after PD-L1 knockdown and HJB (50 µM) treatment; (H) growth of HCC827 cells after PD-L1 knockdown and HJB (50 µM) treatment. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; n.s., not significant. HJB, 17-hydroxy-jolkinolide B; qPCR, quantitative polymerase chain reaction; NC, negative control; PD-L1, programmed death ligand 1; Sh-PD-L1, short hairpin RNA targeting PD-L1; SiPD-L1, small interfering RNA targeting PD-L1; Si-STAT3, small interfering RNA targeting PD-L1; GAPDH, glyceraldehyde phosphate dehydrogenase; IHC, immunohistochemistry; HPA, Human Protein Atlas; NC-HCC827, negative control of small interfering RNA used in HCC827 cells; Si-HCC827, small interfering RNA targeting PD-L1 used in HCC827 cells; NC-Lewis, negative control of short hairpin RNA targeting PD-L1 used in Lewis cells; shRNA-Lewis, short hairpin RNA targeting PD-L1 used in Lewis cells.

Article Snippet: The common-used LUAD cell lines HCC827, H2228, and normal lung fibroblast cells WI38 were obtained from Procell (Cat#CL-0094, Cat#CL-0570, Cat#CM-0466, Wuhan, China) which have passed the STR verification and mycoplasma test by MycAwayTM Mycoplasma Real-time qPCR Detection Kit from YeSen (Cat#40618ES25, Shanghai, China).

Techniques: Expressing, Small Interfering RNA, Western Blot, Knockdown, Real-time Polymerase Chain Reaction, Negative Control, shRNA, Immunohistochemistry