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Image Search Results
Journal: Journal of Thoracic Disease
Article Title: 17-hydroxy-jolkinolide B potentiated CTLA4ab therapy through targeting tumor suppression and immune activation by downregulating PD-L1 expression in lung adenocarcinoma
doi: 10.21037/jtd-24-781
Figure Lengend Snippet: PD-L1 served as a novel target in HJB-suppressed lung cancer. (A) STAT3 expression in HCC827 cells detected by qPCR after HJB (0, 50, 100 µM) treatment; (B) PD-L1 expression in HCC827 cells detected by qPCR after using small interfering RNA targeted STAT3; (C) PD-L1 expression in LUAD cells and normal lung fibroblast cells detected by qPCR; (D) IHC analysis of PD-L1 expression downloaded from the HPA ( www.proteinatlas.org ), scale bar, 200 µm; (E) Western blot analysis of PD-L1 expression after HJB (0, 50, 100 µM) treatment; (F) the efficiency of PD-L1 knockdown detected by qPCR; (G) cell growth of Lewis after PD-L1 knockdown and HJB (50 µM) treatment; (H) growth of HCC827 cells after PD-L1 knockdown and HJB (50 µM) treatment. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; n.s., not significant. HJB, 17-hydroxy-jolkinolide B; qPCR, quantitative polymerase chain reaction; NC, negative control; PD-L1, programmed death ligand 1; Sh-PD-L1, short hairpin RNA targeting PD-L1; SiPD-L1, small interfering RNA targeting PD-L1; Si-STAT3, small interfering RNA targeting PD-L1; GAPDH, glyceraldehyde phosphate dehydrogenase; IHC, immunohistochemistry; HPA, Human Protein Atlas; NC-HCC827, negative control of small interfering RNA used in HCC827 cells; Si-HCC827, small interfering RNA targeting PD-L1 used in HCC827 cells; NC-Lewis, negative control of short hairpin RNA targeting PD-L1 used in Lewis cells; shRNA-Lewis, short hairpin RNA targeting PD-L1 used in Lewis cells.
Article Snippet: The common-used LUAD cell lines HCC827, H2228, and normal lung fibroblast cells WI38 were obtained from Procell (Cat#CL-0094, Cat#CL-0570, Cat#CM-0466, Wuhan, China) which have passed the STR verification and mycoplasma test by
Techniques: Expressing, Small Interfering RNA, Western Blot, Knockdown, Real-time Polymerase Chain Reaction, Negative Control, shRNA, Immunohistochemistry